INVESTIGATION OF THE PRESENCE OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE IN MAMMALIAN HEPATIC PEROXISOMES

被引：0

作者：

PAUL, HS ^{[1
]}

SEKAS, G ^{[1
]}

ADIBI, SA ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH MED,DEPT MOLEC GENET & BIOCHEM,PITTSBURGH,PA 15213

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1992年 / 24卷 / 04期

关键词：

D O I：

10.1016/0020-711X(92)90336-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Rat liver was fractionated into peroxisomes and mitochondria and branched-chain keto acid (BCKA) dehydrogenase activity was measured. 2. All BCKA dehydrogenase activity was associated with the mitochondrial fraction and none with the peroxisomal fraction. 3. BCKA dehydrogenase was also not detected in hepatic peroxisomes of rats treated with clofibrate which induces several peroxisomal enzymes. 4. Hepatic peroxisomes from rabbit, hamster and dog also did not show any BCKA dehydrogenase activity. 5. We conclude that mammalian hepatic peroxisomes do not contain BCKA dehydrogenase.

引用

页码：617 / 619

页数：3

共 21 条

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