MICROGELS AS SOLUBLE SUPPORTS FOR ENZYME ACTIVE AGAINST POLYMERIC SUBSTRATES - MICROCOCCAL NUCLEASE

被引：3

作者：

ALCANTARA, AR

SINISTERRA, JV

GUANTI, G

THEA, S

WILLIAMS, A

机构：

[1] UNIV GENOA,IST CHIM ORGAN,I-16126 GENOA,ITALY

[2] UNIV CANTERBURY,CHEM LABS,CANTERBURY,ENGLAND

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1991年 / 70卷 / 03期

关键词：

D O I：

10.1016/0304-5102(91)80133-N

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Micrococcal nuclease has been conjugated to soluble carboxyl-bearing microgels with a water-soluble carbodiimide. The stability of the enzyme-polymer conjugate is significantly enhanced over that of the native enzyme. The value of K(m) of the immobilised enzyme is raised some 30-fold, probably due to electrostatic repulsion, whereas k(cat) is essentially unchanged.

引用

页码：381 / 389

页数：9

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