2-DIMENSIONAL CRYSTALS OF STREPTAVIDIN ON BIOTINYLATED LIPID LAYERS AND THEIR INTERACTIONS WITH BIOTINYLATED MACROMOLECULES

被引：296

作者：

DARST, SA ^{[1
]}

AHLERS, M ^{[1
]}

MELLER, PH ^{[1
]}

KUBALEK, EW ^{[1
]}

BLANKENBURG, R ^{[1
]}

RIBI, HO ^{[1
]}

RINGSDORF, H ^{[1
]}

KORNBERG, RD ^{[1
]}

机构：

[1] UNIV MAINZ,INST ORGAN CHEM,W-6500 MAINZ,GERMANY

来源：

BIOPHYSICAL JOURNAL | 1991年 / 59卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(91)82232-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free biotin-binding sites on the surface of the two-dimensional crystals facing the aqueous solution. The free biotin-binding sites could be specifically labeled with biotinylated ferritin. The streptavidin/biotinylated lipid system may provide a general approach for the formation of two-dimensional crystals of biotinylated macromolecules.

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页码：387 / 396

页数：10

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