ISOLATION OF A SRC HOMOLOGY 2-CONTAINING TYROSINE PHOSPHATASE

被引:336
作者
PLUTZKY, J
NEEL, BG
ROSENBERG, RD
机构
[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[2] BETH ISRAEL HOSP,MOLEC MED UNIT,BOSTON,MA 02215
关键词
CELL SIGNALING; PROTEIN PHOSPHORYLATION; TYROSINE KINASES; MEGAKARYOCYTES;
D O I
10.1073/pnas.89.3.1123
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Tyrosine phosphorylation is controlled by the opposing actions of tyrosine kinases and phosphotyrosine phosphatases (PTPs). src homology 2 domains (SH2) are found in several types of signaling proteins, including some tyrosine kinases. These domains bind phosphotyrosyl proteins and thus help promote signal transduction. Using mixed oligonucleotide-directed polymerase chain reactions, two previously undescribed rat PTP cDNA fragments were generated. Through subsequent screening of rat megakaryocyte and human erythroleukemia libraries, we obtained a full-length coding sequence for one of these fragments. This cDNA, SH-PTP1, encodes a tyrosine phosphatase containing two highly conserved SH2 domains. SH-PTPI, with a 2.4-kilobase nRNA, a predicted open reading frame of 595 amino acids, and a structure suggesting a nontransmembrane protein, is expressed primarily in hematopoietic and epithelial cells. When expressed in Escherichia coli, SH-PTP1 possesses PTP activity. The structure of SH-PTP1 establishes an additional branch of the tyrosine phosphatase family and suggests mechanisms through which tyrosine phosphatases might participate in signal transduction pathways.
引用
收藏
页码:1123 / 1127
页数:5
相关论文
共 45 条
[1]   MOLECULAR-CLONING AND CHROMOSOME MAPPING OF THE HUMAN GENE ENCODING PROTEIN PHOSPHOTYROSYL PHOSPHATASE-1B [J].
BROWNSHIMER, S ;
JOHNSON, KA ;
LAWRENCE, JB ;
JOHNSON, C ;
BRUSKIN, A ;
GREEN, NR ;
HILL, DE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (13) :5148-5152
[2]   ONCOGENES AND SIGNAL TRANSDUCTION [J].
CANTLEY, LC ;
AUGER, KR ;
CARPENTER, C ;
DUCKWORTH, B ;
GRAZIANI, A ;
KAPELLER, R ;
SOLTOFF, S .
CELL, 1991, 64 (02) :281-302
[3]  
CARTWRIGHT CA, 1987, CELL, V49, P65
[4]   HUMAN-PLACENTA PROTEIN-TYROSINE-PHOSPHATASE - AMINO-ACID SEQUENCE AND RELATIONSHIP TO A FAMILY OF RECEPTOR-LIKE PROTEINS [J].
CHARBONNEAU, H ;
TONKS, NK ;
KUMAR, S ;
DILTZ, CD ;
HARRYLOCK, M ;
COOL, DE ;
KREBS, EG ;
FISCHER, EH ;
WALSH, KA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5252-5256
[5]   CLONING OF A CDNA FOR A MAJOR HUMAN PROTEIN-TYROSINE-PHOSPHATASE [J].
CHERNOFF, J ;
SCHIEVELLA, AR ;
JOST, CA ;
ERIKSON, RL ;
NEEL, BG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2735-2739
[6]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[7]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995
[8]   CDNA ISOLATED FROM A HUMAN T-CELL LIBRARY ENCODES A MEMBER OF THE PROTEIN-TYROSINE-PHOSPHATASE FAMILY [J].
COOL, DE ;
TONKS, NK ;
CHARBONNEAU, H ;
WALSH, KA ;
FISCHER, EH ;
KREBS, EG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (14) :5257-5261
[9]   EXPRESSION OF A HUMAN T-CELL PROTEIN-TYROSINE-PHOSPHATASE IN BABY HAMSTER-KIDNEY CELLS [J].
COOL, DE ;
TONKS, NK ;
CHARBONNEAU, H ;
FISCHER, EH ;
KREBS, EG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) :7280-7284
[10]   TYR527 IS PHOSPHORYLATED IN PP60C-SRC - IMPLICATIONS FOR REGULATION [J].
COOPER, JA ;
GOULD, KL ;
CARTWRIGHT, CA ;
HUNTER, T .
SCIENCE, 1986, 231 (4744) :1431-1434