A RAPID METHOD FOR DETECTION OF YERSINIA-ENTEROCOLITICA SEROTYPE O-3 IN PIG FECES USING MONOCLONAL-ANTIBODIES

被引：6

作者：

LI, QZ ^{[1
]}

DISANTO, ME ^{[1
]}

MAGEE, WE ^{[1
]}

机构：

[1] DREXEL UNIV,DEPT BIOSCI & BIOTECHNOL,32ND & CHESTNUT ST,PHILADELPHIA,PA 19104

来源：

VETERINARY MICROBIOLOGY | 1992年 / 32卷 / 01期

关键词：

D O I：

10.1016/0378-1135(92)90004-D

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A simple colony immunoblotting method using monoclonal antibodies (MAbs) was developed to detect Y. enterocolitica serotype O:3 in pig feces. One of the MAbs studied was able to detect single colonies of the organism in the presence of calculated 3.1 x 10(8) heterologous organisms in pig feces. The MAb was found to be specific for the lipopolysaccharide (LPS) O-antigens of Y. enterocolitica serotype O:3. No significant cross-reactivity was found against a variety of closely related serotypes and Gram-negative organisms. The MAb could also be used in a slide agglutination test and an indirect fluorescence antibody assay for rapid identification of Y enterocolitica serotype O:3.

引用

页码：29 / 41

页数：13

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