MICROBIAL HYDROXYLATION OF 3-CYANOPYRIDINE TO 3-CYANO-6-HYDROXYPYRIDINE

被引：22

作者：

YASUDA, M ^{[1
]}

SAKAMOTO, T ^{[1
]}

SASHIDA, R ^{[1
]}

UEDA, M ^{[1
]}

MORIMOTO, Y ^{[1
]}

NAGASAWA, T ^{[1
]}

机构：

[1] OKAYAMA UNIV, FAC AGR, DEPT BIOL FUNCT & GENET RESOURCES SCI, NAKA, OKAYAMA 700, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 04期

关键词：

D O I：

10.1271/bbb.59.572

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Approximately 4600 microorganisms isolated from soil and 110 bacteria from stock cultures were screened for their ability to hydroxylate 3-cyanopyridine (3-CP). Many strains were found to be capable of hydroxylating 3-CP. One of the strains with the highest activity was selected and identified as Comamonas testosteroni (MCI 2848). The product was isolated and identified as 3-cyano-6-hydroxypyridine (6-CHP). We optimized culture and reaction conditions for the production of 6-CHP by resting cells of MCI 2848. The activity was induced by addition of nicotinic acid or pyridine-3-aldoxime to the culture medium, The optimal temperature and pH of the reaction were 28 degrees C and 7.0, respectively. When resting cells was incubated with 3% (w/v) 3-CP, 95% of the 3-CP was converted into 6-CHP within 47 h.

引用

页码：572 / 575

页数：4

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