A DNA APTAMER WHICH BINDS TO AND INHIBITS THROMBIN EXHIBITS A NEW STRUCTURAL MOTIF FOR DNA

被引：332
作者：
WANG, KY
MCCURDY, S
SHEA, RG
SWAMINATHAN, S
BOLTON, PH
机构：
[1] GILEAD SCI, 346 LAKESIDE DR, FOSTER CITY, CA 94404 USA
[2] WESLEYAN UNIV, DEPT CHEM, MIDDLETOWN, CT 06459 USA
来源：
BIOCHEMISTRY | 1993年 / 32卷 / 08期
关键词：
D O I：
10.1021/bi00059a003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The investigation of the three-dimensional structure of the DNA aptamer d(G1G2T3T4G5G6T7G8T9G10G11T12T13G14G15) which binds to and inhibits thrombin has been carried out by NMR methods. This DNA exhibits a number of long-range NOEs between residues which are not adjacent in sequence, which allowed the determination of the novel tertiary structure adopted. This DNA adopts a highly compact, highly symmetrical structure which consists of two tetrads of guanosine base pairs and three loops. The residues of the tetrads alternate anti-syn-anti-syn. This novel structural motif for DNA may also be relevant to the structure of telomere DNA.
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页码：1899 / 1904
页数：6
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