A DNA APTAMER WHICH BINDS TO AND INHIBITS THROMBIN EXHIBITS A NEW STRUCTURAL MOTIF FOR DNA

被引：332

作者：

WANG, KY

MCCURDY, S

SHEA, RG

SWAMINATHAN, S

BOLTON, PH

机构：

[1] GILEAD SCI, 346 LAKESIDE DR, FOSTER CITY, CA 94404 USA

[2] WESLEYAN UNIV, DEPT CHEM, MIDDLETOWN, CT 06459 USA

来源：

BIOCHEMISTRY | 1993年 / 32卷 / 08期

关键词：

D O I：

10.1021/bi00059a003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The investigation of the three-dimensional structure of the DNA aptamer d(G1G2T3T4G5G6T7G8T9G10G11T12T13G14G15) which binds to and inhibits thrombin has been carried out by NMR methods. This DNA exhibits a number of long-range NOEs between residues which are not adjacent in sequence, which allowed the determination of the novel tertiary structure adopted. This DNA adopts a highly compact, highly symmetrical structure which consists of two tetrads of guanosine base pairs and three loops. The residues of the tetrads alternate anti-syn-anti-syn. This novel structural motif for DNA may also be relevant to the structure of telomere DNA.

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页码：1899 / 1904

页数：6

相关论文

共 27 条

[1] STRUCTURE AND FUNCTION OF TELOMERES [J].

BLACKBURN, EH .

NATURE, 1991, 350 (6319) :569-573

[2] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[3] DETERMINATION OF 3-DIMENSIONAL STRUCTURES OF PROTEINS AND NUCLEIC-ACIDS IN SOLUTION BY NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY [J].

CLORE, GM ;

GRONENBORN, AM .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1989, 24 (05) :479-564

[4] ASSIGNMENT OF RESONANCES IN THE P-31 NUCLEAR MAGNETIC-RESONANCE SPECTRUM OF POLY[D(A-T)] FROM PHOSPHOROTHIOATE SUBSTITUTION [J].

ECKSTEIN, F ;

JOVIN, TM .

BIOCHEMISTRY, 1983, 22 (19) :4546-4550

[5] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

[6]

Ernst R., 1987, PRINCIPLES NMR ONE 2

[7] Z-DNA FORMS WITHOUT AN ALTERNATING PURINE-PYRIMIDINE SEQUENCE IN SOLUTION [J].

FEIGON, J ;

WANG, AHJ ;

VANDERMAREL, GA ;

VANBOOM, JH ;

RICH, A .

SCIENCE, 1985, 230 (4721) :82-84

[8] 4-STRANDED NUCLEIC-ACID STRUCTURES 25 YEARS LATER - FROM GUANOSINE GELS TO TELOMER DNA [J].

GUSCHLBAUER, W ;

CHANTOT, JF ;

THIELE, D .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1990, 8 (03) :491-511

[9] CATION-DEPENDENT TRANSITION BETWEEN THE QUADRUPLEX AND WATSON-CRICK HAIRPIN FORMS OF D(CGCG3GCG) [J].

HARDIN, CC ;

WATSON, T ;

CORREGAN, M ;

BAILEY, C .

BIOCHEMISTRY, 1992, 31 (03) :833-841

[10] MONOVALENT CATION INDUCED STRUCTURAL TRANSITIONS IN TELOMERIC DNAS - G-DNA FOLDING INTERMEDIATES [J].

HARDIN, CC ;

HENDERSON, E ;

WATSON, T ;

PROSSER, JK .

BIOCHEMISTRY, 1991, 30 (18) :4460-4472