EFFECTS OF NERVE GROWTH-FACTOR AND DEXAMETHASONE ON NA+,K+-ATPASE OF CULTURED PC12H CELLS

被引：8

作者：

KURIHARA, K

HOSOI, K

UEHA, T

NAKANISHI, N

YAMADA, S

机构：

[1] MEIKAI UNIV,SCH DENT,DEPT ORAL PHYSIOL,SAKADO,SAITAMA 35002,JAPAN

[2] MEIKAI UNIV,SCH DENT,DEPT ORAL BIOCHEM,SAKADO,SAITAMA 35002,JAPAN

来源：

HORMONE AND METABOLIC RESEARCH | 1994年 / 26卷 / 01期

关键词：

NA+-K+-ATPASE; NGF; PC12H CELLS;

D O I：

10.1055/s-2007-1000765

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

When PC12h cells were cultured for 4 days in the presence of 50 ng/ml of nerve growth factor (NGF), they showed elongated dendrites and specifically increased Na+,K+-ATPase activity. Either singly or in combination with NGF, dexamethasone also increased the specific activity of this enzyme. Western blot analysis using anti-alpha 1 and anti-alpha 2 antisera showed that PC12h cells, either before or after hormone treatment, contained the alpha 1 isoform but not the alpha 2 one. We conclude, therefore, that NGF induces Na+,K+-ATPase concomitantly with neuronal differentiation in PC12h cells but that the growth factor does not induce formation of the myelin sheath, which normally expresses the alpha 2 isoform of Na+,K+-ATPase.

引用

页码：14 / 18

页数：5

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