The aim of this study was to assess the effects of increasing specific (paddling erogmeter) and nonspecific (cycle ergometer) exercise on parameters relating to the ventilatory threshold (Th-vent) and work efficiency in 11 young female flat-water kayakists. When these trained subjects were tested using non-specific workloads, their oxygen uptake (VO2) values at Th-vent, as a percentage of VO2max (% VO2max), were close to those of untrained subjects [74.2 (5.6) % VO2max, mean (SD)]. However, when we tested the same subjects using specific exercise, we recorded values typical of highly trained athletes [84.8 (4.7) % VO2max). For the non-specific exercise on the cycle erogmeter, we recorded work efficiency values close to those of untrained subjects [22.3 (2.5) %]; however, for the specific exercise on the paddling ergometer, we recorded much lower values [13.4 (3.0) %] both at the level of Th-vent. The work efficiency at two warm-up submaximal exercise loads on the paddling ergometer was nonsignificantly lower than values at Th-vent [12.3 (2.8) % and 12.9 (2.9) % respectively]. Significant correlations were found between maximal-performance VO2 (ml.kg(-1).min(-1)) and performance at Th-vent during paddling and race performance (0.623, 0.630 and 0.648 respectively, all P<0.05). Because the results of both specific and non-specific submaximal exercise tests are different, we suggest caution in the interpretation of physiological variables that may be sensitive to training status. The evaluation of Th-vent and work efficiency as supplementary parameters during laboratory studies enables the determination of the effectiveness of the training process and the specific adaptation of the subjects.