OBSERVING THE H-1-NMR SIGNAL OF THE MYOGLOBIN VAL-E11 IN MYOCARDIUM - AN INDEX OF CELLULAR OXYGENATION

被引：65

作者：

KREUTZER, U ^{[1
]}

WANG, DS ^{[1
]}

JUE, T ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT BIOL CHEM,DAVIS,CA 95616

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 10期

关键词：

D O I：

10.1073/pnas.89.10.4731

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The H-1 NMR signal from oxymyoglobin, a low-concentration diamagnetic protein, is visible in myocardial tissue. The methyl group of the Val-E11 resonates in a clear spectral region at -2.76 ppm and responds to dynamic changes in cellular oxygenation. With CO, the signal shifts to -2.4 ppm. The Val-E11 peak assignment and its response to oxygen and CO agree perfectly with previous myoglobin solution studies. Intracellular oxygen level can now be determined in vivo with the signal intensity ratio of oxymyoglobin/deoxymyoglobin, reflected by the Val-E11 and His-F8 peaks in the H-1 NMR spectra. Moreover, protein structure-function relationship in vivo can now be probed.

引用

页码：4731 / 4733

页数：3

共 17 条

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