BIOCHEMICAL-EVIDENCE FOR THE AUTOPHOSPHORYLATION AND TRANSPHOSPHORYLATION OF TRANSFORMING GROWTH-FACTOR-BETA RECEPTOR KINASES

被引：115

作者：

CHEN, F

WEINBERG, RA

机构：

[1] WHITEHEAD INST BIOMED RES, CAMBRIDGE, MA 02142 USA

[2] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 05期

关键词：

TRANSMEMBRANE SERINE; THREONINE KINASES; PHOSPHORYLATION;

D O I：

10.1073/pnas.92.5.1565

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transforming growth factor beta (TGF-beta) signals through a receptor complex containing the type I (TGF-beta RI) and type II (TGF-beta RII) receptors. We describe here biochemical studies on early events in the TGF-beta signaling pathway. TGF-beta RII is highly phosphorylated when expressed alone in COS-1 cells; its autophosphorylation occurs via an intramolecular (cis) mechanism that is independent of ligand binding. TGF-beta RI is also highly phosphorylated when expressed alone in COS-I cells. Both wild-type TGF-beta RI and a kinase-deficient mutant thereof are transphosphorylated by the coexpressed TGF-beta RII kinase in a ligand-independent fashion in these cells. We propose that the association of TGF-beta RI and TGF-beta RII, induced by ligand binding or overexpression, leads to transphosphorylation of the TGF-beta RI by the TGF-beta RII kinase. This represents a mechanism of activation of receptors distinct from that of tyrosine kinase receptors and may apply to other serine/threonine kinase receptors.

引用

页码：1565 / 1569

页数：5

共 31 条

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