Amplification and Sequencing of 16/18S rDNA from Gel-Purified Total Plant DNA

被引：52

作者：

Bult, Carol ^{[1
]}

Kaellersjoe, Mari ^{[1
]}

Suh, Youngbae ^{[1
]}

机构：

[1] Natl Museum Nat Hist, Smithsonian Inst, Lab Mol Systemat, Washington, DC 20560 USA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1992年 / 10卷 / 03期

关键词：

DNA extraction; gel purification; PCR amplification; double-stranded DNA sequencing; rDNA; nuclear; chloroplast;

D O I：

10.1007/BF02668360

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here methods and strategies for amplifying and sequencing the genes encoding the small subunits (16/18S) of nuclear and chloroplast ribosomal DNA (rDNA) from total plant DNA. These methods were developed in response to technical difficulties we encountered in our molecular systematic work with members of various plant families. These protocols have proved useful when the amount of tissue available for study is limited and when the tissues have high concentrations of undesirable secondary metabolites which are often co-isolated with nucleic acids.

引用

页码：273 / 284

页数：12

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