THE DSBA-DSBB SYSTEM AFFECTS THE FORMATION OF DISULFIDE BONDS IN PERIPLASMIC BUT NOT IN INTRAMEMBRANOUS PROTEIN DOMAINS

被引：15

作者：

WHITLEY, P ^{[1
]}

VONHEIJNE, G ^{[1
]}

机构：

[1] KAROLINSKA INST,CTR STRUCT BIOCHEM,NOVUM,DEPT MOLEC BIOL,S-14157 HUDDINGE,SWEDEN

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

MEMBRANE PROTEIN; DISULFIDE; PROTEIN EXPORT; LEADER PEPTIDASE;

D O I：

10.1016/0014-5793(93)80481-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DsbA and DsbB proteins of Escherichia coli are involved in facilitating the formation of disulfide bonds in periplasmic proteins. Here, we show that the rate of formation of a disulfide bond in the periplasmic domain of the inner membrane protein leader peptidase is reduced in dsbA and dsbB strains, whereas the rate of formation of a disulfide bond engineered into the.membrane embedded domain of the same protein is completely unaffected by these mutations. We conclude that the Dsb proteins do not facilitate the formation of intramembraneous disulfides.

引用

页码：49 / 51

页数：3

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