Enhancing the resistance to H2S toxicity during anaerobic digestion of low-strength wastewater through granular activated carbon (GAC) addition

Low-strength wastewater was treated using two laboratory-scale up-flow anaerobic sludge blankets (UASB) for 130 days under sulfate-reducing conditions. Granular activated carbon (GAC) was added to one of the reactors. The GAC addition increased the total chemical oxygen demand removal by 21-28% and total methane production by 32-78%. The sludge from the GAC-amended UASB showed higher specific methanogenic activities (SMA) and higher activities in the presence of H2S, indicating that the GAC addition enhanced the resistance of methanogens to H2S toxicity. Further, the microbial communities showed that the GAC addition shifted microbial communities. A robust syntrophic partnership between bacteria (i.e., Bacteroidetes_vadinHA17 and Trichococcus) and methanogens was established in the GAC-amended UASB. Sulfate-reducing bacteria (SRB) were enriched in the GAC biofilm, indicating the coexistence of competition and cooperation between SRB and methanogens. These findings provide significant insights regarding microbial community dynamics, especially SRB and methanogens, in a GAC-amended anaerobic digestion process under sulfate-reducing conditions.