A COMPARISON OF THE LEAKAGE OF A MONOCLONAL-ANTIBODY FROM VARIOUS IMMUNOAFFINITY CHROMATOGRAPHY MATRICES

被引：0

作者：

BEER, DJ

YATES, AM

RANDLES, SC

JACK, GW

机构：

[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DIV PROD,SALISBURY SP4 0JG,WILTS,ENGLAND

[2] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DIV QUAL SAFETY & SCI RESOURCES,SALISBURY SP4 0JG,WILTS,ENGLAND

来源：

BIOSEPARATION | 1995年 / 5卷 / 04期

关键词：

IMMUNOAFFINITY CHROMATOGRAPHY; LEAKAGE; MONOCLONAL ANTIBODY;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibody leakage from immunoaffinity chromatography (IAC) matrices could reduce the working life of the IAC matrix and/or contaminate parenteral products, purified by IAC. There is therefore a need to measure the leakage of antibody from IAC matrices and to reduce such leakage. Using sensitive ELISAs it was found that the type of activated matrix, the buffer, the presence of proteases in the feedstock and the storage of IAC matrices between runs could all effect antibody leakage.

引用

页码：241 / 247

页数：7

共 18 条

[1] ELUTION CONDITIONS AND DEGRADATION MECHANISMS IN LONG-TERM IMMUNOADSORBENT USE [J].