APPLICATION OF A NEW STRUCTURED MODEL TO TOBACCO CELL-CULTURES

被引：15

作者：

HOOKER, BS ^{[1
]}

LEE, JM ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,DEPT CHEM ENGN,PULLMAN,WA 99164

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 07期

关键词：

PLANT CELLS; SUSPENSION CULTURE; STRUCTURED KINETIC MODEL; TOBACCO CELL CULTURES;

D O I：

10.1002/bit.260390709

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new structured kinetic model has been formulated and applied to batch suspensions of Nicotiana tabacum. This model has been developed by representing culture interactions with pathways designated for structural component production, secondary metabolite synthesis, and cellular respiration. Additional provisions were made to distinguish growth-competitive secondary metabolite production from non-growth-competitive secondary metabolite production. Parameters for kinetic rate expressions within the model were estimated based upon experimental observations utilized in conjunction with numerical optimization techniques. Using these parameters, culture growth, substrate uptake, cell respiration, and total phenolics production were all successfully correlated to experimental data from shake flask cultures of N. tabacum.

引用

页码：765 / 774

页数：10

共 28 条

[11]

LEE JM, 1982, BIOCH ENG

[12]

LEUDEKING R, 1959, J BIOCH MICROBIOL TE, V1, P393

[13] ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES [J].