MOLECULAR-CLONING AND EXPRESSION OF A NEW ALPHA-SUBUNIT OF SOLUBLE GUANYLYL CYCLASE - INTERCHANGEABILITY OF THE ALPHA-SUBUNITS OF THE ENZYME

被引：148

作者：

HARTENECK, C ^{[1
]}

WEDEL, B ^{[1
]}

KOESLING, D ^{[1
]}

MALKEWITZ, J ^{[1
]}

BOHME, E ^{[1
]}

SCHULTZ, G ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST PHARMAKOL,THIELALLEE 67-73,W-1000 BERLIN 33,GERMANY

来源：

FEBS LETTERS | 1991年 / 292卷 / 1-2期

关键词：

SOLUBLE GUANYLYL CYCLASE; CDNA; AMINO ACID SEQUENCE; EXPRESSION;

D O I：

10.1016/0014-5793(91)80871-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA coding for a new subunit of soluble guanylyl cyclase with a calculated molecular mass of 81.7 kDa was cloned and sequenced. On the basis of sequence homology, the new subunit appears to be an isoform of the alpha-1-subunit and was designated alpha-2 as the new subunit is very similar to the alpha-1-subunit in the middle and C-terminal part; it is quite diverse in the N-terminal part. Preceding experiments had shown that coexpression of the alpha-1- and beta-1-subunits is necessary to obtain a catalytically active guanylyl cyclase in COS cells [(1990) FEBS Lett. 272, 221-223]. The finding that the alpha-2-subunit was able to replace the alpha-1- but not the beta-1-subunit in expression experiments demonstrates the interchangeability of the alpha-subunit isoforms of soluble guanylyl cyclase.

引用

页码：217 / 222

页数：6

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