AMINOACYL TRANSFER RIBONUCLEIC ACID SYNTHETASES FROM CELL-FREE EXTRACT OF PLASMODIUM BERGHEI

被引：13

作者：

ILAN, J

机构：

[1] Department of Biology, Temple University, Philadelphia

来源：

SCIENCE | 1969年 / 164卷 / 3879期

关键词：

D O I：

10.1126/science.164.3879.560

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aminoacyl transfer ribonucleic acid synthetases for leucine, tyrosine, histidine, valine, proline, threonine, and lysine were obtained from cell-free extract of Plasmodium berghei. The leucyl-tRNA synthetase can charge tRNA from liver or Escherichia coli with leucine-C14, liver tRNA being a better substrate. The amount of aminoacylation increases linearly with respect to the quantity of tRNA added from either source and is dependent on the amount of enzyme added. The rate of aminoacylation is constant for 10 minutes and then decreases. It is enhanced by polyvinylsulfate. One-tenth millimolar pyrimethamine, hydroxystilbamidine, quinacrine, and acriflavine inhibited the formation of C14-valyl-tRNA. Species specificity between tRNA and its charging enzyme with respect to the recognition site is discussed.

引用

页码：560 / +

共 11 条

[1]

DOCTOR BP, 1963, J BIOL CHEM, V238, P3677

[2] SPECIES SPECIFICITY OF TYROSINE TRANSFER RNAS FROM ESCHERICHIA COLI AND YEAST [J].

HAYASHI, H .

JOURNAL OF MOLECULAR BIOLOGY, 1966, 19 (01) :161-&

[3]

ILAN J, 1968, FED PROC, V27, P798

[4]

ILAN J, 1967, FED PROC, V26, P448

[5]

ILAN J, 1966, ACTA BIOCHIM POL, V13, P353

[6]

ILAN J, 1968, J BIOL CHEM, V236, P1372

[7]

NOVELLI D, 1967, ANNU REV BIOCHEM, V36, P649

[8]

SADUN EH, DA1767C7158 US ARM C

[9]

TOKUYASU K, IN PRESS

[10] COMMENTS ON ULTRASTRUCTURE [J].

TRAGER, W .

MILITARY MEDICINE, 1966, 131 (9S) :1009-&

← 1 2 →