DETERMINATION OF THE LENGTH DISTRIBUTION OF POLY(A) AT THE 3' TERMINUS OF THE VIRION RNAS OF EMC VIRUS, POLIOVIRUS, RHINOVIRUS, RAV-61 AND CPMV AND OF MOUSE GLOBIN MESSENGER-RNA

被引：79
作者：
AHLQUIST, P [1 ]
KAESBERG, P [1 ]
机构：
[1] UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BIOCHEM,MADISON,WI 53706
来源：
NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 05期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1093/nar/7.5.1195
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rapid, detailed, and accurate analysis of the length spectrum of 3′ terminal poly(A) in an RNA population can be obtained by 3′-terminal 32P-labelllng of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3′-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented. © 1979 Information Retrieval Limited.
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页码：1195 / 1204
页数：10
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