DETERMINATION OF THE LENGTH DISTRIBUTION OF POLY(A) AT THE 3' TERMINUS OF THE VIRION RNAS OF EMC VIRUS, POLIOVIRUS, RHINOVIRUS, RAV-61 AND CPMV AND OF MOUSE GLOBIN MESSENGER-RNA

被引：79

作者：

AHLQUIST, P ^{[1
]}

KAESBERG, P ^{[1
]}

机构：

[1] UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BIOCHEM,MADISON,WI 53706

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.5.1195

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid, detailed, and accurate analysis of the length spectrum of 3′ terminal poly(A) in an RNA population can be obtained by 3′-terminal 32P-labelllng of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3′-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented. © 1979 Information Retrieval Limited.

引用

页码：1195 / 1204

页数：10

共 28 条

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