IMPROVED METHOD FOR THE RAPID ISOLATION OF BRAIN RIBONUCLEIC-ACID

被引：109
|
作者：
KAPLAN, BB
BERNSTEIN, SL
GIOIO, AE
机构：
来源：
BIOCHEMICAL JOURNAL | 1979年 / 183卷 / 01期
关键词：
D O I：
10.1042/bj1830181
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An improved one-step method for the extraction of RNA from rat brain is described. Fresh or frozen tissue is disrupted in the powerful protein denaturant guanidine thiocyanate, and RNA isolated by ultracentrifugation through CsCl. The procedure is advantageous in that it is relatively simple, is rapid and does not expose the sample to enzyme treatments or repeated organic extractions.
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页码：181 / 184
页数：4
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