LIQUID-CHROMATOGRAPHIC SEPARATION AND STEREOSELECTIVE DETECTION OF L-AMINO AND D-AMINO ACIDS WITH CATALYTIC REACTION DETECTION USING IMMOBILIZED ENZYMES

被引：15

作者：

DOMINGUEZ, E ^{[1
]}

MARKOVARGA, G ^{[1
]}

CARLSSON, M ^{[1
]}

GORTON, L ^{[1
]}

机构：

[1] UNIV LUND,DEPT ANALYT CHEM,S-22100 LUND,SWEDEN

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 8-12期

关键词：

D-AMINO ACID OXIDASE; L-AMINO ACID OXIDASE; IMMOBILIZED ENZYME REACTOR; LIQUID CHROMATOGRAPHY; D-AMINO AND L-AMINO ACIDS; SERUM;

D O I：

10.1016/0731-7085(90)80127-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A post-column LC detection system is described for the stereoselective detection of L- and D-amino acids. The effluent of the LC column passes an immobilized enzyme reactor (IMER) containing either L- or D-amino acid oxidase. The hydrogen peroxide formed in this reactor by the oxidation of the amino acids is then transported to a second IMER containing horse-radish peroxidase. With the addition of 4-aminophenazone and dichlorophenolsulphonyl chloride to the carrier, the hydrogen peroxide is reacted to form a red-coloured complex which is detected in a flow-through photometric cell at 514 nm. Applications to the analysis of amino acids in bovine and human sera are described.

引用

页码：825 / 830

页数：6

共 35 条

[1] ORTHO-PHTHALALDEHYDE DERIVATIVES OF AMINES FOR HIGH-SPEED LIQUID-CHROMATOGRAPHY ELECTROCHEMISTRY [J].