STRUCTURE OF LACTATE-DEHYDROGENASE INHIBITOR GENERATED FROM COENZYME

被引：21

作者：

BIELLMANN, JF ^{[1
]}

LAPINTE, C ^{[1
]}

HAID, E ^{[1
]}

WEIMANN, G ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH,BIOCHEM WERK,D-8132 TUTZING,FED REP GER

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 07期

关键词：

D O I：

10.1021/bi00574a015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two inhibitors of lactate dehydrogenase generated during NADH storage have been isolated by chromatography. One is a dimer of the dinucleotide where the AMP moiety is unmodified. The other is also generated from NAD+ in the presence of a high concentration of phosphate ions at alkaline pH. This inhibitor was proved to be the addition compound of one phosphate group to position C-4 of the nicotinamide ring of NAD+ by NMR spectroscopy, enzymatic cleavage, and dissociation to NAD+ at neutral pH. This compound is a competitive inhibitor with respect to NAD+ in the presence of the lactate dehydrogenase with a Ki of 2X10-7 M. The interaction of this inhibitor with lactate dehydrogenase is discussed relative to the structure of this enzyme. © 1979, American Chemical Society. All rights reserved.

引用

页码：1212 / 1217

页数：6

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