Development of DNA-immobilised chromatographic stationary phases for optical resolution and DNA-affinity comparison of metal complexes

被引：37

作者：

AldrichWright, JR

Greguric, I

Vagg, RS

Vickery, K

Williams, PA

机构：

[1] MACQUARIE UNIV,SCH CHEM,SYDNEY,NSW 2109,AUSTRALIA

[2] UNIV WESTERN SYDNEY NEPEAN,DEPT CHEM,KINGSWOOD,NSW 2747,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 718卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/0021-9673(95)00703-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two chiral stationary phases which exploit DNA as a chiral discriminator have been developed. A covalently-bound DNA stationary phase for HPLC applications was used to optically resolve [Ru(dipyrido[6,7-d:2',3'-f]-quinoxaline)(3)](2+) and [Ru(1,10-phenanthroline)(3)](2+) complex ions. This shows that the column retention times are influenced both by pH and by the size of the aromatic ligands. DNA-immobilised on cellulose paper proved effective for simultaneously comparing the relative retention of a number of metal complexes and R(F) data correlate well with the degree of aromatic area in the complexes available for intercalation into DNA.

引用

页码：436 / 443

页数：8

共 43 条

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[2]

ALDRICHWRIGHT JR, 1993, THESIS MACQUARIE U

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