DISTRIBUTION AND CHARACTERIZATION OF IMMUNOREACTIVE RAT ADRENOMEDULLIN IN TISSUE AND PLASMA

被引：264

作者：

SAKATA, J ^{[1
]}

SHIMOKUBO, T ^{[1
]}

KITAMURA, K ^{[1
]}

NISHIZONO, M ^{[1
]}

IEHIKI, Y ^{[1
]}

KANGAWA, K ^{[1
]}

MATSUO, H ^{[1
]}

ETO, T ^{[1
]}

机构：

[1] NATL CARDIOVASC CTR,RES INST,SUITA,OSAKA 565,JAPAN

来源：

FEBS LETTERS | 1994年 / 352卷 / 02期

关键词：

ADRENOMEDULLIN (RAT); RADIOIMMUNOASSAY; DISTRIBUTION; CHARACTERIZATION; PLASMA; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;

D O I：

10.1016/0014-5793(94)00928-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adrenomedullin is a new bioactive peptide recently isolated from pheochromocytoma. We report on the rat adrenomedullin distribution and molecular forms in various tissues and plasma. Using a sensitive radioimmunoassay system for rat adrenomedullin, high concentrations of immunoreactive rat adrenomedullin were detected in adrenal gland, lung and cardiac atrium. In lung and atrium, the immunoreactivity concentration in rat was about 6-10 times higher than that in human. The mean plasma concentration of immunoreactive rat adrenomedullin was 3.60 +/- 0.34 fmol/ml (mean +/- S.D.). Analysis in adrenal gland, lung and atrium with reverse-phase and gel-filtration high-performance liquid chromatography showed that most immunoreactive rat adrenomedullin emerged as a single peak at a position exactly identical to that of the authentic rat adrenomedullin peptide, synthesized according to the sequence predicted from the cDNA.

引用

页码：105 / 108

页数：4

共 9 条

[1] LABELING OF PROTEINS TO HIGH SPECIFIC RADIOACTIVITIES BY CONJUGATION TO A I-125-CONTAINING ACYLATING AGENT - APPLICATION TO RADIOIMMUNOASSAY [J].