MICROMACHINING OF CAPILLARY ELECTROPHORESIS INJECTORS AND SEPARATORS ON GLASS CHIPS AND EVALUATION OF FLOW AT CAPILLARY INTERSECTIONS

被引：365

作者：

FAN, ZH ^{[1
]}

HARRISON, DJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 01期

关键词：

D O I：

10.1021/ac00073a029

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capillary electrophoresis system with a sample injector has been integrated on a 1 X 2 cm planar glass chip by use of micromachining techniques. Separation of three amino acids was achieved in the device within 4 s. A maximum of 6800 theoretical plates was obtained with 470 V between injection and detection points. Electric fields of 2500 V/cm and power dissipation of 2.3 W/m did not result in Joule heating effects due to the thermal mass and conductivity of the glass chips. Leakage at channel intersection points was found to arise from convective as well as diffusion effects. Methods of device fabrication are presented.

引用

页码：177 / 184

页数：8

共 24 条

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Chapman A. J., 1987, FUNDAMENTALS HEAT TR, P14

[2]

Coulson J. M., 1977, CHEM ENG, V1

[3] INFLUENCE OF ANALYTE PLUG WIDTH ON PLATE NUMBER IN CAPILLARY ELECTROPHORESIS [J].