SIMULTANEOUS DETERMINATION OF A NEW GASTROINTESTINAL PROKINETIC AGENT (HSR-803) AND ITS METABOLITES IN HUMAN SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING AUTOMATED COLUMN-SWITCHING

被引：12

作者：

TAKAHARA, E

FUKUOKA, H

TAKAGI, T

NAGATA, O

KATO, H

机构：

[1] Central Research Laboratories, Hokuriku Seiyaku Co., Ltd., Katsuyama-shi, Fukui

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 576卷 / 01期

关键词：

D O I：

10.1016/0378-4347(92)80191-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method based on high-performance liquid chromatography using column-switching is described for the simultaneous determination of HSR-803 and its metabolites in human serum and urine. The system uses a six-port valve with a Nucleosil CN pre-column for on-line sample clean-up, and direct injection of samples. The limits of quantitation in serum and urine were 5 and 20 ng/ml for HSR-803 and 50 and 200 ng/ml for the metabolites, respectively. The coefficients of variation for the intra- and inter-day accuracies were between 0.8 and 7.1% for each compound. This method was applied to the pharmacokinetic studies in humans after oral administration of HSR-803.

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页码：174 / 178

页数：5

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