A KINETIC ANALYSIS OF COUPLED ENZYME ASSAYS

被引：218

作者：

MCCLURE, WR

机构：

[1] Department of Biochemistry, Institute for Enzyme Research, University of Wisconsin, Madison, Wisconsin

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 07期

关键词：

D O I：

10.1021/bi00835a014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rate equations for consecutive irreversible reactions such as are encountered in coupled enzyme assays are solved. For an assay containing one auxiliary enzyme, an expression is derived relating the time required to produce a linear reaction velocity and the amount of coupling enzyme added. The more complicated system in which two auxiliary enzymes are used required a graphical analysis. A nomogram is presented which shows the dependence of each coupling enzyme on the other in producing a linear reaction rate. Practical considerations for implementing these results are discussed with a view toward conserving auxiliary enzymes, saving the investigator's time, and increasing his confidence in results obtained from coupled assays. © 1969, American Chemical Society. All rights reserved.

引用

页码：2782 / &