IDENTIFICATION OF THE TARGET OF A TRANSCRIPTION ACTIVATOR PROTEIN BY PROTEIN-PROTEIN PHOTO-CROSS-LINKING

被引:109
作者
CHEN, Y
EBRIGHT, YW
EBRIGHT, RH
机构
[1] RUTGERS STATE UNIV, DEPT CHEM, NEW BRUNSWICK, NJ 08855 USA
[2] RUTGERS STATE UNIV, WAKSMAN INST, NEW BRUNSWICK, NJ 08855 USA
关键词
D O I
10.1126/science.8016656
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Here it is shown, with the use of protein-protein photocrosslinking, that the carboxyl-terminal region of the alpha subunit of RNA polymerase (RNAP) is in direct physical proximity to the activating region of the catabolite gene activator protein (CAP) in the ternary complex of the lac promoter, RNAP, and CAP, These results strongly support the proposal that transcription activation by CAP involves protein-protein contact between the carboxyl-terminal region of the alpha subunit and the activating region of CAP.
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收藏
页码:90 / 92
页数:3
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