FLOW-CYTOMETRY - INSTRUMENTATION AND APPLICATION IN PHYTOPLANKTON RESEARCH

被引：18

作者：

BALFOORT, HW

BERMAN, T

MAESTRINI, SY

WENZEL, A

ZOHARY, T

机构：

[1] KINNERET LIMNOL LAB, IL-14102 TIBERIAS, ISRAEL

[2] CTR RECH ECOL MARINE & AQUACULTURE HOUMEAU, CNRS, IFREMER, F-171137 HOUMEAU, FRANCE

[3] FRAUNHOFER INST ENVIRONM CHEM & ECOTOXICOL, W-5948 SCHMALLENBERG, GERMANY

来源：

HYDROBIOLOGIA | 1992年 / 238卷

关键词：

D O I：

10.1007/BF00048777

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

In flow cytometry, light scattering and fluorescence of individual particles in suspension is measured at high speed. When applied to planktonic particles, the light scattering and (auto-)fluorescence properties of algal cells can be used for cell identification and counting. Analysis of the wide size spectrum of phytoplankton species, generally present in eutrophic inland and coastal waters, requires flow cytometers specially designed for this purpose. This paper compares the performance in phytoplankton research of a commercial flow cytometer to a purpose built instrument. It reports on the identification of phytoplankton and indicates an area where flow cytometry may supersede more conventional techniques: the analysis of morphological and physiological characteristics of subpopulations in phytoplankton samples.

引用

页码：89 / 97

页数：9

共 17 条

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