ISOLATION AND CHARACTERIZATION OF A YEAST CHROMOSOMAL REPLICATOR

被引：603

作者：

STINCHCOMB, DT

STRUHL, K

DAVIS, RW

机构：

[1] Department of Biochemistry, Stanford University, School of Medicine, Stanford

来源：

NATURE | 1979年 / 282卷 / 5734期

关键词：

D O I：

10.1038/282039a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication. © 1979 Nature Publishing Group.

引用

页码：39 / 43

页数：5

共 32 条

[1] CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].