PURIFICATION AND PROPERTIES OF NUCLEAR AND CYTOPLASMIC DEOXYRIBONUCLEIC ACID LIKE RIBONUCLEIC ACID FROM EHRLICH ASCITES CELLS

Newly synthesized ribonucleic acid was extracted from the nuclear and cytoplasmic fractions of Ehrlich ascites cells. These ribonucleic acids were fractionated further by chromatography on methylated albumin kieselguhr columns. Methods were developed whereby the ribonucleic acid rich in adenylic acid and uridylic acid (“deoxyribonucleic acid like” ribonucleic acid) from the nucleus and cytoplasm could be isolated from methylated albumin kieselguhr columns in a highly purified form. The cytoplasmic deoxyribonucleic acid like ribonucleic acid was found to differ significantly from the nuclear deoxyribonucleic acid like ribonucleic acid in both sedimentation properties and in base composition. This provides additional evidence that within animal cells there exists a species of ribonucleic acid which is restricted to the nucleus. © 1969, American Chemical Society. All rights reserved.