VIRUS-MEDIATED OR TRANSGENIC SUPPRESSION OF A G-PROTEIN ALPHA-SUBUNIT AND ATTENUATION OF FUNGAL VIRULENCE

被引：123

作者：

CHOI, GH ^{[1
]}

CHEN, BS ^{[1
]}

NUSS, DL ^{[1
]}

机构：

[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,NUTLEY,NJ 07110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 01期

关键词：

CRYPHONECTRIA PARASITICA; HYPOVIRULENCE; SIGNAL TRANSDUCTION; TRANSGENIC COSUPPRESSION; BIOLOGICAL CONTROL;

D O I：

10.1073/pnas.92.1.305

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the G(i) class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal strains. Significantly, the resulting transgenic fungal strains were also hypovirulent. These results indicate a crucial role for G-protein-linked signal transduction in fungal pathogenesis and suggest a molecular basis for virus-mediated attenuation of fungal virulence.

引用

页码：305 / 309

页数：5