VIRUS-MEDIATED OR TRANSGENIC SUPPRESSION OF A G-PROTEIN ALPHA-SUBUNIT AND ATTENUATION OF FUNGAL VIRULENCE

被引：123

作者：

CHOI, GH ^{[1
]}

CHEN, BS ^{[1
]}

NUSS, DL ^{[1
]}

机构：

[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,NUTLEY,NJ 07110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 01期

关键词：

CRYPHONECTRIA PARASITICA; HYPOVIRULENCE; SIGNAL TRANSDUCTION; TRANSGENIC COSUPPRESSION; BIOLOGICAL CONTROL;

D O I：

10.1073/pnas.92.1.305

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the G(i) class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal strains. Significantly, the resulting transgenic fungal strains were also hypovirulent. These results indicate a crucial role for G-protein-linked signal transduction in fungal pathogenesis and suggest a molecular basis for virus-mediated attenuation of fungal virulence.

引用

页码：305 / 309

页数：5

共 25 条

[21] ISOLATION AND CHARACTERIZATION OF A GENE ENCODING A G-PROTEIN ALPHA SUBUNIT FROM SCHIZOSACCHAROMYCES-POMBE - INVOLVEMENT IN MATING AND SPORULATION PATHWAYS
OBARA, T
NAKAFUKU, M
YAMAMOTO, M
KAZIRO, Y
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) : 5877 - 5881
[22] ALZHEIMERS-DISEASE - SPECIFIC INCREASES IN A G-PROTEIN SUBUNIT (GS-ALPHA) MESSENGER-RNA IN HIPPOCAMPAL AND CORTICAL-NEURONS
HARRISON, PJ
BARTON, AJL
MCDONALD, B
PEARSON, RCA
MOLECULAR BRAIN RESEARCH, 1991, 10 (01): : 71 - 81
[23] Rice heterotrimeric G-protein alpha subunit (RGA1): In silico analysis of the gene and promoter and its upregulation under abiotic stress
Yadav, Dinesh K.
Shukla, Devesh
Tuteja, Narendra
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2013, 63 : 262 - 271
[24] Phosphorylation of phosducin-like protein BDM-1 by protein kinase 2 (CK2) is required for virulence and Gβ subunit stability in the fungal plant pathogen Cryphonectria parasitica
Salamon, Joanna A.
Acuna, Rachel
Dawe, Angus L.
MOLECULAR MICROBIOLOGY, 2010, 76 (04) : 848 - 860
[25] Interaction of the heterotrimeric G protein alpha subunit SSG-1 of Sporothrix schenckii with proteins related to stress response and fungal pathogenicity using a yeast two-hybrid assay
Perez-Sanchez, Lizaida
Gonzalez, Elizabeth
Colon-Lorenzo, Emilee E.
Gonzalez-Velazquez, Waleska
Gonzalez-Mendez, Ricardo
Rodriguez-del Valle, Nuri
BMC MICROBIOLOGY, 2010, 10

← 1 2 3 →