VIRUS-MEDIATED OR TRANSGENIC SUPPRESSION OF A G-PROTEIN ALPHA-SUBUNIT AND ATTENUATION OF FUNGAL VIRULENCE

被引：123
作者：
CHOI, GH [1 ]
CHEN, BS [1 ]
NUSS, DL [1 ]
机构：
[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,NUTLEY,NJ 07110
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 01期
关键词：
CRYPHONECTRIA PARASITICA; HYPOVIRULENCE; SIGNAL TRANSDUCTION; TRANSGENIC COSUPPRESSION; BIOLOGICAL CONTROL;
D O I：
10.1073/pnas.92.1.305
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the G(i) class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal strains. Significantly, the resulting transgenic fungal strains were also hypovirulent. These results indicate a crucial role for G-protein-linked signal transduction in fungal pathogenesis and suggest a molecular basis for virus-mediated attenuation of fungal virulence.
引用
收藏
页码：305 / 309
页数：5
相关论文
共 37 条
[1]   HYPOVIRULENCE CONVERSION IN ENDOTHIA-PARASITICA [J].
ANAGNOSTAKIS, SL ;
DAY, PR .
PHYTOPATHOLOGY, 1979, 69 (12) :1226-1229
[2]   BIOLOGICAL-CONTROL OF CHESTNUT BLIGHT [J].
ANAGNOSTAKIS, SL .
SCIENCE, 1982, 215 (4532) :466-471
[3]  
Bavendamm W., 1928, Z PFLANZENKRANKH, V38, P257
[4]   MYRISTOYLATED ALPHA-SUBUNITS OF GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEINS [J].
BUSS, JE ;
MUMBY, SM ;
CASEY, PJ ;
GILMAN, AG ;
SEFTON, BM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7493-7497
[5]   INHIBITION OF GENE-EXPRESSION BY A SHORT SENSE FRAGMENT [J].
CAMERON, FH ;
JENNINGS, PA .
NUCLEIC ACIDS RESEARCH, 1991, 19 (03) :469-475
[6]   ATTENUATION OF FUNGAL VIRULENCE BY SYNTHETIC INFECTIOUS HYPOVIRUS TRANSCRIPTS [J].
CHEN, BS ;
CHOI, GH ;
NUSS, DL .
SCIENCE, 1994, 264 (5166) :1762-1764
[7]   MITOTIC STABILITY AND NUCLEAR INHERITANCE OF INTEGRATED VIRAL CDNA IN ENGINEERED HYPOVIRULENT STRAINS OF THE CHESTNUT BLIGHT FUNGUS [J].
CHEN, BS ;
CHOI, GH ;
NUSS, DL .
EMBO JOURNAL, 1993, 12 (08) :2991-2998
[8]   HYPOVIRULENCE OF CHESTNUT BLIGHT FUNGUS CONFERRED BY AN INFECTIOUS VIRAL CDNA [J].
CHOI, GH ;
NUSS, DL .
SCIENCE, 1992, 257 (5071) :800-803
[9]   A VIRAL GENE CONFERS HYPOVIRULENCE-ASSOCIATED TRAITS TO THE CHESTNUT BLIGHT FUNGUS [J].
CHOI, GH ;
NUSS, DL .
EMBO JOURNAL, 1992, 11 (02) :473-477
[10]   MOLECULAR ANALYSIS OF THE LACCASE GENE FROM THE CHESTNUT BLIGHT FUNGUS AND SELECTIVE SUPPRESSION OF ITS EXPRESSION IN AN ISOGENIC HYPOVIRULENT STRAIN [J].
CHOI, GH ;
LARSON, TG ;
NUSS, DL .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1992, 5 (02) :119-128
1234