THE KINETICS OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE IN-VIVO INFERRED FROM MEASUREMENTS OF PHOTOSYNTHESIS IN LEAVES OF TRANSGENIC TOBACCO

Transgenic tobacco (Nicotiana tabacum L. cv. W38) with an antisense gene directed against the mRNA of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit was used to determine the kinetic properties of Rubisco in vivo. The leaves of these plants contained only 34% as much Rubisco as those of the wild type, but other photosynthetic components were not significantly affected. Consequently, the rate of CO2 assimilation by the antisense plants was limited by Rubisco activity over a wide range of CO2 partial pressures. Unlike in the wild-type leaves, where the rate of regeneration of ribulose bisphosphate limited CO2 assimilation at intercellular partial pressures above 400 mu bar, photosynthesis in the leaves of the antisense plants responded hyperbolically to CO2, allowing the kinetic parameters of Rubisco in vivo to be inferred. We calculated a maximal catalytic turnover rate, k(cat), of 3.5+/-0.2 mol CO2.(mol sites)(-1).s(-1) at 25 degrees C in vivo. By comparison, we measured a value of 2.9 mol CO2.(mol sites)(-1).s(-1) in vitro with leaf extracts. To estimate the Michaelis-Menten constants for CO2 and O-2, the rate of CO2 assimilation was measured at 25 degrees C at different intercellular partial pressures of CO2 and O-2. These measurements were combined with carbon-isotope analysis (C-13/C-12) of CO2 in the air passing over the leaf to estimate the conductance for transfer of CO2 from the substomatal cavities to the sites of carboxylation (0.3 mol m(-2).s(-1).bar(-1)) and thus the partial pressure of CO2 at the sites of carboxylation. The calculated Michaelis-Menten constants for CO2 and O-2 were 259+/-57 mu bar (8.6+/-1.9 mu M) and 179 mbar (226 mu M), respectively, and the effective Michaelis-Menten constant for CO2 in 200 mbar O-2 was 549 mu bar (18.3 mu M). From measurements of the photocompensation point (Gamma* = 38.6 mu bar) we estimated Rubisco's relative specificity for CO2, as opposed to O-2 to be 97.5 in vivo. These values were dependent on the size of the estimated CO2-transfer conductance.