GLYCOPROTEIN STRUCTURE - CARBOHYDRATE OF BOVINE CORNEAL COLLAGEN

被引：15
作者：
BOSMANN, HB
JACKSON, JJ
机构：
[1] The Salk Institute for Biological Studies, La Jolla, CA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 170卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/0304-4165(68)90156-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. 1. Compositional studies using gas-liquid chromatography performed on bovine corneal collagen indicated that this molecule is a glycoprotein with a carbohydrate content of 5.78%. 2. 2. The sugar components present were identified as glucosamine, 0.47%; galactosamine, 3.08%; fucose, 0.02%; mannose, 0.21%; xylose, 0.31%; glucose 0.57%; galactose, 1.09%; and sialic acid 0.05%. 3. 3. Digestion of the corneal collagen with collagenase and pronase, and column chromatography yields two distinct glycopeptides with differing carbohydrate composition. © 1968.
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页码：6 / &
相关论文
共 22 条
[1]   COLLAGEN-GLUCOSYL TRANSFERASE IN FIBROBLASTS TRANSFORMED BY ONCOGENIC VIRUSES [J].
BOSMANN, HB ;
EYLAR, EH .
NATURE, 1968, 218 (5141) :582-&
[2]   ATTACHMENT OF CARBOHYDRATE TO COLLAGEN . ISOLATION PURIFICATION AND PROPERTIES OF GLUCOSYL TRANSFERASE [J].
BOSMANN, HB ;
EYLAR, EH .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 30 (01) :89-&
[3]  
BUTLER WT, 1965, J BIOL CHEM, V240, P3449
[4]  
BUTLER WT, 1966, J BIOL CHEM, V241, P3882
[5]  
DISCHE Z, 1947, J BIOL CHEM, V167, P189
[6]   CHEMICAL STUDIES ON DESCEMET MEMBRANE OF THE BOVINE CORNEA [J].
DOHLMAN, CH ;
BALAZS, EA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1955, 57 (02) :445-457
[7]   CONTINUOUS AMINO ACID ANALYSIS - ELUTION PROGRAMMING AND AUTOMATIC COLUMN SELECTION BY MEANS OF A ROTATING VALVE [J].
DUS, K ;
LINDROTH, S ;
PABST, R ;
SMITH, RM .
ANALYTICAL BIOCHEMISTRY, 1966, 14 (01) :41-+
[8]  
EYLAR EH, 1965, J THEOR BIOL, V10, P89
[9]   ISOLATION AND AMINO ACID COMPOSITION OF BOVINE CORNEAL POLYMERIC COLLAGENS [J].
FREEMAN, IL ;
STEVEN, FS ;
JACKSON, DS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 154 (01) :252-&
[10]   ANALYSIS OF NEUTRAL SUGARS BY GAS-LIQUID CHROMATOGRAPHY OF ALDITOL ACETATES - APPLICATION TO THYROTROPIC HORMONE AND OTHER GLYCOPROTEINS [J].
KIM, JH ;
SHOME, B ;
LIAO, TH ;
PIERCE, JG .
ANALYTICAL BIOCHEMISTRY, 1967, 20 (02) :258-+
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