RESEARCH TECHNIQUES FOR ESTIMATING PHENOTYPIC AND GENOTYPIC DIVERSITY OF ROOT-AND STEM-NODULE BACTERIA

被引：0

作者：

Pongsilp, Neelawan ^{[1
]}

Boonkerd, Nantakorn ^{[2
]}

机构：

[1] Silpakorn Univ, Fac Sci, Dept Microbiol, Sanam Chandra Palace Campus, Amphoe Muang 73000, Nakhon Pathom, Thailand

[2] Suranaree Univ Technol, Inst Agr Technol, Sch Biotechnol, Nakhon Ratchasima 30000, Thailand

来源：

SURANAREE JOURNAL OF SCIENCE AND TECHNOLOGY | 2007年 / 14卷 / 03期

关键词：

Diversity; Root- and stem-nodule bacteria; numerical analysis; RAPD; rep-PCR; AFLP; RFLP; PCR-RFLP;

D O I：

暂无

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Root- and stem-nodule bacteria symbiotically nodulate and fix nitrogen with certain leguminous plants. Members in 14 genera have been reported as nitrogen-fixing symbionts. Phenotypic and genotypic diversity of these symbiotic bacteria have been revealed, providing valuable information, through the use of methodologies for phylogenetic analyses. Numerical analysis based on phenotypic characteristics and techniques based on DNA analysis, including 16S rDNA sequence analyis, RAPD, rep-PCR, AFLP, RFLP and PCR-RFLP, have been widely used for this purpose. A brief overview of these techniques is required to evaluate their suitability, discriminating power and limitation.

引用

页码：297 / 308

页数：12

共 36 条

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