ALLOTRIPLOID SOMATIC HYBRIDS OF DIPLOID TOMATO (LYCOPERSICON-ESCULENTUM MILL) AND MONOPLOID POTATO (SOLANUM-TUBEROSUM L)

被引：12
|
作者：
SCHOENMAKERS, HCH [1 ]
WOLTERS, AMA [1 ]
NOBEL, EM [1 ]
DEKLEIN, CMJ [1 ]
KOORNNEEF, M [1 ]
机构：
[1] WAGENINGEN UNIV AGR, DEPT GENET, DREIJENLAAN 2, 6703 HA WAGENINGEN, NETHERLANDS
来源：
THEORETICAL AND APPLIED GENETICS | 1993年 / 87卷 / 03期
关键词：
LYCOPERSICON-ESCULENTUM; SOLANUM-TUBEROSUM; ALLOTRIPLOID SOMATIC HYBRIDS; NITRATE REDUCTASE DEFICIENCY;
D O I：
10.1007/BF01184919
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Allotriploid somatic hybrids were obtained from fusions between protoplasts of diploid tomato and monohaploid potato. The selection of fusion products was carried out in two different ways: (1) The fusion of nitrate reductase-deficient tomato with potato gave rise only to hybrid calli if selection was performed on media lacking ammonium. Parental microcalli were rarely obtained and did not regenerate. (2) The fusion of cytoplasmic albino tomato with potato gave rise to albino and green hybrid calli and plants. Allotriploids were identified from the two somatic hybrid populations by counting chloroplast numbers in leaf guard cells and by flow cytometry of leaf tissue. Although some pollen fertility of allotriploids and pollen-tube growth of tomato, potato and Lycopersicon pennellii into the allotriploid style were observed, no progeny could be obtained. The relevance of allotriploid somatic hybrids in facilitating limited gene transfer from potato to tomato is discussed.
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页码：328 / 336
页数：9
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