REFINED SOLUTION STRUCTURE OF THE DIMERIC QUADRUPLEX FORMED FROM THE OXYTRICHA TELOMERIC OLIGONUCLEOTIDE D(GGGGTTTTGGGG)

被引:155
作者
SCHULTZE, P [1 ]
SMITH, FW [1 ]
FEIGON, J [1 ]
机构
[1] UNIV CALIF LOS ANGELES, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA
关键词
DNA STRUCTURE; G-DNA; GUANINE QUARTET; QUADRUPLEX; 2-DIMENSIONAL NMR;
D O I
10.1016/S0969-2126(00)00023-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Telomeres, the structures at the ends of linear eukaryotic chromosomes, are essential for chromosome replication and stability. The telomeres of the unicellular ciliate Oxytricha contain a 3' single strand overhang composed of two repeats of the telomere repeat sequence d(TTTTGGGG). It has been proposed that oligonucleotides containing this repeat can form DNA quadruplexes via hydrogen bonding of the guanines into quartets. Such structures may be relevant to the biological function of the telomere, and in G-rich sequences elsewhere in the genome. Results: We have previously determined from solution NMR data that the Oxy-1.5 Oxytricha repeat oligonucleotide d(GGGGTTTTGGGG) dimerizes to form an intermolecular quadruplex composed of four guanine quartets and with the thymines in loops across the diagonal at opposite ends of the quadruplex. We report here the refined solution structure of Oxy-1.5. This structure is compared with the previously published crystal structure of the same oligonucleotide. Conclusions: Oxy-1.5 forms a well-defined, symmetrical structure with ordered thymine loops. Both the solution and crystal structures of Oxy-1.5 are quadruplexes with alternating syn and anti glycosyl conformation of guanines along each strand of the helix and have thymine loops at opposite ends. However, the topology of the two structures is fundamentally different, leading to significant structural differences. A topological pathway for the formation and interconversion of the two structures is proposed.
引用
收藏
页码:221 / 233
页数:13
相关论文
共 64 条
[1]   NMR-STUDY OF PARALLEL-STRANDED TETRAPLEX FORMATION BY THE HEXADEOXYNUCLEOTIDE D(TG4T) [J].
ABOULELA, F ;
MURCHIE, AIH ;
LILLEY, DMJ .
NATURE, 1992, 360 (6401) :280-282
[2]   THE COHERENCE OF SYNTHETIC TELOMERES [J].
ACEVEDO, OL ;
DICKINSON, LA ;
MACKE, TJ ;
THOMAS, CA .
NUCLEIC ACIDS RESEARCH, 1991, 19 (12) :3409-3419
[3]   QUADRUPLEX DNA FORMATION IN A REGION OF THE TRANSFER-RNA GENE SUPF ASSOCIATED WITH HYDROGEN-PEROXIDE MEDIATED MUTATIONS [J].
AKMAN, SA ;
LINGEMAN, RG ;
DOROSHOW, JH ;
SMITH, SS .
BIOCHEMISTRY, 1991, 30 (35) :8648-8653
[4]   STRUCTURES FOR POLYINOSINIC ACID AND POLYGUANYLIC ACID [J].
ARNOTT, S ;
CHANDRASEKARAN, R ;
MARTTILA, CM .
BIOCHEMICAL JOURNAL, 1974, 141 (02) :537-+
[5]   THE NUCLEIC-ACID DATABASE - A COMPREHENSIVE RELATIONAL DATABASE OF 3-DIMENSIONAL STRUCTURES OF NUCLEIC-ACIDS [J].
BERMAN, HM ;
OLSON, WK ;
BEVERIDGE, DL ;
WESTBROOK, J ;
GELBIN, A ;
DEMENY, T ;
HSIEH, SH ;
SRINIVASAN, AR ;
SCHNEIDER, B .
BIOPHYSICAL JOURNAL, 1992, 63 (03) :751-759
[6]  
BLACKBURN EH, 1990, J BIOL CHEM, V265, P5919
[7]   TELOMERASES [J].
BLACKBURN, EH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :113-129
[8]   STRUCTURE AND FUNCTION OF TELOMERES [J].
BLACKBURN, EH .
NATURE, 1991, 350 (6319) :569-573
[9]   SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].
BOCK, LC ;
GRIFFIN, LC ;
LATHAM, JA ;
VERMAAS, EH ;
TOOLE, JJ .
NATURE, 1992, 355 (6360) :564-566
[10]  
BORZO M, 1978, CR ACAD SCI C CHIM, V287, P475