EFFECT OF SUPPLEMENTING DIFFERENT LEVELS AND SOURCES OF PHYTASE ENZYME TO THE LAYING HENS DIETS ON PRODUCTIVE PERFORMANCE

This study was conducted in the Poultry Research Station - State Board of Agricultural Research - Ministry of Agricultural in Abu Ghraib, to study the effect of supplementing different levels and sources of Phytase enzyme to laying hens diets on productive performance and quality of eggs produced. Nine hundred sixty egg-brown hens (Lohmann Brown-Classic) 24 weeks-old distributed randomly to six treatments with various sources of phytase, each source contain three levels which include 16 treatments with 2 replicates (30 hen per replicate). Chickens fed on diets equal protein and metabolic energy according to the age periods in Lohmann Index as in follow: Treatment 1 (T1): Control group (without any supplement and without reduction of calcium and phosphorus). Treatment 2 (T2): Supplement phytase from fungal source (Aspergillus oryza). Treatment 3 (T3): Supplement phytase from bacterial source (E. coli). Treatment 4 (T4): Supplement phytase from phytase mixture For every three source of phytase added 3 levels as follows: A: a diet reduced phosphorus (0.09) and calcium (0.07) which included Phytase adding by 250 FTY / kg feed B: a diet reduced phosphorus (0.12) and calcium (0.10) which included Phytase adding by 350 FTY / kg feed C: a diet reduced phosphorus (0.15) and calcium (0.13) which included Phytase adding by 450 FTY / kg feed Treatment 5 (T5): Supplement Phytase from yeast (Saccharomyces cerevisia). Treatment 6 (T6): Supplement Phytase from Alfalfa plant. For every two source of Phytase (T5 and T6) added 3 levels 2.5, 3.5 and 4.5 FTY for each source. All three levels diet reduced phosphorus and calcium as in T2, T3 and T4. The results of experiment showed: Significant differences (P<0.05) between treatments in accumulative egg production (HD%), egg weight, egg mass and feed conversion ratio, while the adding of T2 treatment at level (A, C) and T4 treatment at level (C) were highly significant than other treatments. All treatments with adding Phytase enzyme showed significant superiority (P<0.05) for qualitative characteristics of eggs produced from 24-55 weeks as follows. From this study we can concluded that supplementing fungal phytase at levels 250 and 450 FTY/kg feed in layer diets with decrease phosphore 0.09 and 0.15 % and calcium 0.07 and 0.13% respectively gave the best results also when supplementing mixture enzyme at level 450 FTY/kg feed.