INVOLVEMENT OF THE COPPER IN THE INHIBITION OF CU,ZN SUPEROXIDE-DISMUTASE ACTIVITY AT HIGH PH

被引：4
作者：
CALABRESE, L
POLTICELLI, F
CAPO, C
MUSCI, G
机构：
[1] UNIV ROME LA SAPIENZA,DEPT BIOCHEM SCI,PIAZZALE A MORO 5,I-00185 ROME,ITALY
[2] UNIV ROME LA SAPIENZA,CNR,CTR MOLEC BIOL,I-00185 ROME,ITALY
来源：
FREE RADICAL RESEARCH COMMUNICATIONS | 1991年 / 12-3卷
关键词：
D O I：
10.3109/10715769109145799
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The alkaline spectroscopic transition of the copper at the active site of Cu, Zn superoxide dismutase has been reexamined by room temperature EPR. in order to correlate it with the inhibition of the enzyme activity at high pH. The EPR transition is governed by a single prototropic equilibrium, with pK values of 11.3 and 11.1 for ox and shark superoxide dismutase. respectively. This result suggests possible contributions of changes of the copper environment to the higher pK of the activity/pH curve. When Arg141 was cheniically modified by phenylglyoxal treatment of the ox protein. a lower pK value (10.8) was obtained, indicating that Arg141 is involved in the observed modifications of the EPR spectra. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：305 / 312
页数：8
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