DIGOXIGENIN AS A PROBE LABEL FOR INSITU HYBRIDIZATION ON SKELETAL TISSUES

被引：19

作者：

BLAND, YS ^{[1
]}

CRITCHLOW, MA ^{[1
]}

ASHHURST, DE ^{[1
]}

机构：

[1] ST GEORGE HOSP,SCH MED,DEPT ANAT,LONDON SW17 0RE,ENGLAND

来源：

HISTOCHEMICAL JOURNAL | 1991年 / 23卷 / 09期

关键词：

D O I：

10.1007/BF01042298

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Non-specific staining was encountered using digoxigenin-labelled cDNA probes for in situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.

引用

页码：415 / 418

页数：4