In silico Binding and Interaction Studies of Inflammatory Mediators in Isoniazid and Rifampicin Induced Toxicity against Vitamin B12 and Beta-Carotene

Isoniazid (INH) and rifampicin (RIF) form the backbone of anti-tuberculosis treatment but their use leads to hepatotoxicity in a very small percentage of patients. We in our current study would like to screen cytokines, proteins and receptors mediating inflammation and apoptosis by in silico methods to predict the mechanism by which hepatotoxicity is induced. We would also like to look at the mechanism by which previously reported hepatoprotective natural compounds function against INH and RIF-induced inflammation and apoptosis. The pdb structures of chosen cytokines and other inflammatory mediators are to be retrieved from protein data bank and those of the chosen ligands are to be generated using services on Corina molecular networks. Docking studies are to be done on PatchDock online server and analyzed on PyMol molecular viewer. The results obtained would help guide further in vitro and in vivo studies to know the exact mechanism of action of both toxic and protective drugs.