SPECIFIC INDUCTION OF UNCOILING AND RECOMBINATION BY AZACYTIDINE IN CLASSICAL SATELLITE-CONTAINING CONSTITUTIVE HETEROCHROMATIN

被引：84
作者：
KOKALJVOKAC, N
ALMEIDA, A
VIEGASPEQUIGNOT, E
JEANPIERRE, M
MALFOY, B
DUTRILLAUX, B
机构：
[1] INST CURIE,CNRS,URA 620,BIOL SECT,26 RUE ULM,F-75231 PARIS,FRANCE
[2] INST PATHOL MOLEC,PARIS,FRANCE
来源：
CYTOGENETICS AND CELL GENETICS | 1993年 / 63卷 / 01期
关键词：
D O I：
10.1159/000133492
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Azacytidine (ACR) is known to induce uncoiling and somatic association involving the constitutive heterochromatin of human chromosomes 1, 9, 15, and 16 and the Y. These regions are composed of alphoid and classical satellite DNA sequences. Using specific probes for chromosomes 1 and 16, we have performed two-color fluorescence in situ hybridization on human lymphocytes cultured in the presence of ACR. We demonstrate that for these two chromosomes (1) uncoiling and association specifically occur in classical satellite-containing regions at the first cell generation, (2) breakages also affect these regions, and (3) somatic recombinations occur between these regions and lead to translocations at the next cell generation. These results suggest that changes in methylation of repetitive DNA sequences are related to chromosomal instability occurring during cell transformation and tumorigenesis.
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页码：11 / &
相关论文
共 32 条
[11]   DIFFERENTIAL INHIBITION OF SISTER CHROMATID CONDENSATION INDUCED BY 5-AZADEOXYCYTIDINE IN HUMAN-CHROMOSOMES [J].
HAAF, T ;
OTT, G ;
SCHMID, M .
CHROMOSOMA, 1986, 94 (05) :389-394
[12]   ORGANIZATION OF A REPETITIVE HUMAN 1.8 KB KPNI SEQUENCE LOCALIZED IN THE HETEROCHROMATIN OF CHROMOSOME-15 [J].
HIGGINS, MJ ;
WANG, H ;
SHTROMAS, I ;
HALIOTIS, T ;
RODER, JC ;
HOLDEN, JJA ;
WHITE, BN .
CHROMOSOMA, 1985, 93 (01) :77-86
[13]   SV40-TRANSFORMED HUMAN-FIBROBLASTS EXHIBIT A CHARACTERISTIC CHROMOSOMAL PATTERN [J].
HOFFSCHIR, F ;
RICOUL, M ;
DUTRILLAUX, B .
CYTOGENETICS AND CELL GENETICS, 1988, 49 (04) :264-268
[14]   THE ORGANIZATION OF 2 RELATED SUBFAMILIES OF A HUMAN TANDEMLY REPEATED DNA IS CHROMOSOME SPECIFIC [J].
JEANPIERRE, M ;
WEIL, D ;
GALLANO, P ;
CREAUGOLDBERG, N ;
JUNIEN, C .
HUMAN GENETICS, 1985, 70 (04) :302-310
[15]  
JEANPIERRE M, 1987, CYTOGENET CELL GENET, V46, P634
[16]  
JONES PA, 1981, NUCLEIC ACIDS RES, V9, P2933, DOI 10.1093/nar/9.12.2933
[17]   CELLULAR-DIFFERENTIATION, CYTIDINE ANALOGS AND DNA METHYLATION [J].
JONES, PA ;
TAYLOR, SM .
CELL, 1980, 20 (01) :85-93
[18]   A SIMPLE METHOD FOR SIMULTANEOUS R-BANDING OR G-BANDING AND FLUORESCENCE INSITU HYBRIDIZATION OF SMALL SINGLE-COPY GENES [J].
LEMIEUX, N ;
DUTRILLAUX, B ;
VIEGASPEQUIGNOT, E .
CYTOGENETICS AND CELL GENETICS, 1992, 59 (04) :311-312
[19]  
LI LH, 1970, CANCER RES, V30, P2760
[20]   HUMAN CHROMOSOME-SPECIFIC REPETITIVE DNA-SEQUENCES - NOVEL MARKERS FOR GENETIC-ANALYSIS [J].
MOYZIS, RK ;
ALBRIGHT, KL ;
BARTHOLDI, MF ;
CRAM, LS ;
DEAVEN, LL ;
HILDEBRAND, CE ;
JOSTE, NE ;
LONGMIRE, JL ;
MEYNE, J ;
SCHWARZACHERROBINSON, T .
CHROMOSOMA, 1987, 95 (06) :375-386
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