HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF 3-METHYLINDOLE (SKATOLE) AND INDOLE IN ADIPOSE-TISSUE OF PIGS

被引:34
作者
DEHNHARD, M [1 ]
CLAUS, R [1 ]
HILLENBRAND, M [1 ]
HERZOG, A [1 ]
机构
[1] UNIV HOHENHEIM, TIERHALTUNG 470, POB 700562, W-7000 STUTTGART 70, GERMANY
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 616卷 / 02期
关键词
D O I
10.1016/0378-4347(93)80387-J
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A rapid method for the determination of skatole (3-methylindole) and indole in adipose tissue of pigs by reversed-phase high-performance liquid chromatography has been developed. Tissue samples were melted in a microwave oven, and 100 mul of the liquid fat were dissolved in 1 ml of n-hexane and extracted with acetonitrile-water (75:25, v/v). Portions of 100 mul of the solution were used for chromatographic analysis. Elution was performed on a reversed-phase column with a mobile phase composed of acetic acid and isopropanol (70:30, v/v). A fluorescence detector was used for quantification. The detection limit was 4 ng/g fat. The mean recoveries of added amounts of skatole and indole were 98.9 and 93.8%, respectively. The mean coefficients of variation were: inter-assay, 6.6% (skatole) and 8.8% (indole); intra-assay, 4.2% (skatole) and 2.9% (indole). Mean skatole concentrations in fat samples from boars (40 ng/g; n = 349) were not significantly higher than those from barrows (24 ng/g; n = 98).
引用
收藏
页码:205 / 209
页数:5
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