DETECTION OF SOMATIC DNA RECOMBINATION IN THE TRANSGENIC MOUSE-BRAIN

被引：104

作者：

MATSUOKA, M

NAGAWA, F

OKAZAKI, K

KINGSBURY, L

YOSHIDA, K

MULLER, U

LARUE, DT

WINER, JA

SAKANO, H

机构：

[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,DIV NEUROBIOL,BERKELEY,CA 94720

[2] INST PHYS & CHEM RES,TSUKUBA LIFE SCI CTR,IBARAKI 305,JAPAN

[3] BASEL INST IMMUNOL,CH-4005 BASEL,SWITZERLAND

来源：

SCIENCE | 1991年 / 254卷 / 5028期

关键词：

D O I：

10.1126/science.1925563

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A DNA construct containing the bacterial beta-galactosidase gene (lacZ) was used to study somatic DNA recombination in the transgenic mouse brain. Recombination-positive areas of the adult brain were stained blue with X-gal, a substrate of beta-galactosidase. Blue-colored cells appeared soon after birth, and continued to emerge in postnatal tissue. Staining was prominent in sensory as opposed to motor regions of the brain, and was present in more than 70 discrete areas of the nervous system. The possibility of DNA rearrangement is discussed with respect to the development of the central nervous system.

引用

页码：81 / 86

页数：6

共 26 条

[1] 2 PAIRS OF RECOMBINATION SIGNALS ARE SUFFICIENT TO CAUSE IMMUNOGLOBULIN-V-(D)-J JOINING [J].