CARBOXYLESTERASES (EC 3.1.1) . A LARGE-SCALE PURIFICATION OF PIG LIVER CARBOXYLESTERASE

被引：58

作者：

HORGAN, DJ

STOOPS, JK

WEBB, EC

ZERNER, B

机构：

[1] Department of Biochemistry, University of Queensland, St. Lucia

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 05期

关键词：

D O I：

10.1021/bi00833a033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two procedures for the large-scale purification of pig liver carboxylesterase are described. They start from chloroform-acetone powders of minced pig liver and involve ammonium sulfate fractionation, chromatography on CM-cellulose and CM-Sephadex, and gel filtration. These procedures produce an enzyme of hitherto unobtained purity. The yield of enzyme is about 250 mg from 800 g of powder. © 1969, American Chemical Society. All rights reserved.

引用

页码：2000 / &

共 35 条

[1]

ADLER AJ, 1961, J BIOL CHEM, V236, P3240

[2] KINETICS OF PIG LIVER ESTERASE CATALYSIS [J].