ALTERNATIVE PRE-RIBOSOMAL-RNA PROCESSING PATHWAYS IN HUMAN-CELLS AND THEIR ALTERATION BY CYCLOHEXIMIDE INHIBITION OF PROTEIN-SYNTHESIS

被引:87
作者
HADJIOLOVA, KV [1 ]
NICOLOSO, M [1 ]
MAZAN, S [1 ]
HADJIOLOV, AA [1 ]
BACHELLERIE, JP [1 ]
机构
[1] UNIV TOULOUSE 3, CNRS, BIOL MOLEC EUCARYOTE LAB, 118 ROUTE NARBONNE, F-31062 TOULOUSE, FRANCE
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 212卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1993.tb17652.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
rRNA processing pathways in humans have been reinvestigated through systematic Northern-blot hybridizations of HeLa cell nuclear RNA with a collection of digoxigenine-labeled rDNA probes from different regions of the human rDNA transcriptional unit. In addition to the known 45S, 41 S, 32 S and 21 S pre-rRNA, two major pre-rRNA fractions were identified; a '30 S' (about 5800 nucleotides) precursor to 18S rRNA containing an external transcribed spacer at the 5' end (ETS) and internal transcribed spacer (ITS) 1 sequences and a '12S' (about 950 nucleotides) precursor to 5.8S rRNA containing ITS 2 sequences. These pre-rRNA species do not react with probes located near the 3'-terminal segments of ITS 1 or ITS 2, thus suggesting that processive endonuclease cuts occur within ITS spacer sequences. The simultaneous occurrence of at least two alternative 45 S pre-rRNA processing pathways is deduced, which correspond to a different temporal order of endonuclease attack at the sites located near the 5' end of 18S rRNA and within ITS 1. In-vivo labeling experiments with [C-14]uridine revealed that inhibition of protein synthesis with cycloheximide abolishes the endonuclease cut at the 5' end of 18 S rRNA and the formation of 41 S pre-rRNA, while the cut within ITS 1 and the processing to 32S and '30S' pre-rRNA remains relatively unaltered.
引用
收藏
页码:211 / 215
页数:5
相关论文
共 50 条
[11]   GAR1 IS AN ESSENTIAL SMALL NUCLEOLAR RNP PROTEIN REQUIRED FOR PRE-RIBOSOMAL-RNA PROCESSING IN YEAST [J].
GIRARD, JP ;
LEHTONEN, H ;
CAIZERGUESFERRER, M ;
AMALRIC, F ;
TOLLERVEY, D ;
LAPEYRE, B .
EMBO JOURNAL, 1992, 11 (02) :673-682
[12]   THE SMALL NUCLEOLAR RNP PROTEIN NOP1 (FIBRILLARIN) IS REQUIRED FOR PRE-RIBOSOMAL-RNA PROCESSING IN YEAST [J].
TOLLERVEY, D ;
LEHTONEN, H ;
CARMOFONSECA, M ;
HURT, EC .
EMBO JOURNAL, 1991, 10 (03) :573-583
[13]   REGULATION OF PROTEIN-SYNTHESIS IN HUMAN-CELLS EXPOSED TO EXTREME HYPOXIA [J].
KRAGGERUD, SM ;
SANDVIK, JA ;
PETTERSEN, EO .
ANTICANCER RESEARCH, 1995, 15 (03) :683-686
[14]   COLD SHOCK INDUCTION OF YEAST NSR1 PROTEIN AND ITS ROLE IN PRE-RIBOSOMAL-RNA PROCESSING [J].
KONDO, K ;
KOWALSKI, LRZ ;
INOUYE, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1992, 267 (23) :16259-16265
[15]   RELATIONS BETWEEN NUCLEOLAR MORPHOMETRIC PARAMETERS AND PRE-RIBOSOMAL-RNA SYNTHESIS IN ANIMAL AND PLANT-CELLS [J].
NIN, GHV ;
ECHEVERRIA, OM ;
ZAVALA, G ;
JIMENEZGARCIA, LF ;
GONZALEZ, MA ;
PARRA, R .
ACTA ANATOMICA, 1986, 126 (03) :141-146
[16]   REVERSIBLE INHIBITION OF DNA, RNA AND PROTEIN-SYNTHESIS IN HUMAN CELLS BY LEAD CHLORIDE [J].
SKREB, Y ;
HABAZINNOVAK, V .
TOXICOLOGY, 1975, 5 (02) :167-174
[17]   INITIATION AND ELONGATION OF PROTEIN-SYNTHESIS IN GROWING CELLS - DIFFERENTIAL INHIBITION BY CYCLOHEXIMIDE AND EMETINE [J].
OLEINICK, NL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1977, 182 (01) :171-180
[18]   THE SYNTHESIS OF RIBOSOMAL 5S RNA IN CULTURED HAMSTER-CELLS DURING THE INHIBITION OF PROTEIN-SYNTHESIS [J].
TAKENAKA, K ;
YAMAMOTO, M ;
ENDO, H ;
KUWANO, M .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 655 (01) :26-33
[19]   INHIBITION OF DNA, RNA AND PROTEIN-SYNTHESIS AND CHROMATIN ALTERATION BY N-HYDROXYPHENACETIN [J].
HAYWARD, NK ;
LAVIN, MF .
XENOBIOTICA, 1987, 17 (01) :115-124
[20]   DIFFERENTIAL EFFECTS OF CYCLOHEXIMIDE AND OTHER INHIBITORS OF PROTEIN-SYNTHESIS ON INVIVO RIBOSOMAL-RNA SYNTHESIS IN ACHLYA-BISEXUALIS [J].
TIMBERLAKE, WE ;
GRIFFIN, DH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 353 (02) :248-252