DETERMINATION OF ALFUZOSIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN-SWITCHING

被引：14
作者：
CARLUCCI, G
DIGIUSEPPE, E
MAZZEO, P
机构：
[1] Dipartimento di Chimica, Ingegneria Chimica e Materiali, Università dell' Aquila
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 18期
关键词：
D O I：
10.1080/10826079408016168
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic method for the determination of alfuzosin in human plasma has been developed and validated. A column-switching procedure without extraction was used to isolate the drug from biological matrix prior to the quantitative analysis. The lower limit of detection for the analyte was 1 ng/ml. The method was linear from 2 to 150 ng/ml for human plasma. Within- and between-assay precision and accuracy were all found to be <5.2% at the eight concentrations evaluated. This procedure, simple and rapid, is suitable for pharmacology studies on alfuzosin.
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页码：3989 / 3997
页数：9
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