SEQUENCE-ANALYSIS OF THE HN GENE OF PARAINFLUENZA VIRUS TYPE-2

被引：16

作者：

PRECIOUS, B ^{[1
]}

SOUTHERN, JA ^{[1
]}

RANDALL, RE ^{[1
]}

机构：

[1] UNIV ST ANDREWS,DEPT BIOCHEM & MICROBIOL,N ST,ST ANDREWS KY16 9AL,FIFE,SCOTLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-5-1163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA library was constructed in λgt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with 32P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin-neuraminidase (HN) gene were identified by sequence comparisons with known parainfluenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43% amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses.

引用

页码：1163 / 1168

页数：6

共 34 条

[1] HUMAN PARA-INFLUENZA TYPE-3 VIRUS HEMAGGLUTININ-NEURAMINIDASE GLYCOPROTEIN - NUCLEOTIDE-SEQUENCE OF MESSENGER-RNA AND LIMITED AMINO-ACID-SEQUENCE OF THE PURIFIED PROTEIN [J].

ELANGO, N ;

COLIGAN, JE ;

JAMBOU, RC ;

VENKATESAN, S .

JOURNAL OF VIROLOGY, 1986, 57 (02) :481-489

[2] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[3] MONOCLONAL-ANTIBODIES AGAINST HUMAN PARAMYXOVIRUS TYPE-3 AND AGAINST SV5-VIRUS - PREPARATION AND PRELIMINARY CHARACTERIZATION [J].

GOSWAMI, KKA ;

RUSSELL, WC .

JOURNAL OF GENERAL VIROLOGY, 1983, 64 (AUG) :1663-1672

[4] ANTIBODIES AGAINST THE PARAMYXOVIRUS SV5 IN THE CEREBROSPINAL FLUIDS OF SOME MULTIPLE-SCLEROSIS PATIENTS [J].

GOSWAMI, KKA ;

RANDALL, RE ;

LANGE, LS ;

RUSSELL, WC .

NATURE, 1987, 327 (6119) :244-247

[5] DOES SIMIAN VIRUS-5 INFECT HUMANS [J].

GOSWAMI, KKA ;

LANGE, LS ;

MITCHELL, DN ;

CAMERON, KR ;

RUSSELL, WC .

JOURNAL OF GENERAL VIROLOGY, 1984, 65 (AUG) :1295-1303

[6] COLONY HYBRIDIZATION - METHOD FOR ISOLATION OF CLONED DNAS THAT CONTAIN A SPECIFIC GENE [J].

GRUNSTEIN, M ;

HOGNESS, DS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (10) :3961-3965

[7]

HIEBERT SW, 1985, J VIROL, V54, P1

[8] CELL-SURFACE EXPRESSION AND ORIENTATION IN MEMBRANES OF THE 44-AMINO-ACID SH PROTEIN OF SIMIAN VIRUS-5 [J].

HIEBERT, SW ;

RICHARDSON, CD ;

LAMB, RA .

JOURNAL OF VIROLOGY, 1988, 62 (07) :2347-2357

[9]

HSIUNG GD, 1972, PROG MED VIROL, V14, P241

[10]

HYUNH TV, 1985, DNA CLONING TECHNIQU, P49

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